Part-time Research Assistant in Botany/Plant Pathology
5 days ago
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Part-time Research Assistant in Botany/Plant Pathology, DublinClient: Trinity College Dublin
Location: Dublin, Ireland
Job Category: Other
EU work permit required: Yes
Job Reference: 57d6d6e93756
Job Views: 4
Posted: 24.03.2025
Expiry Date: 08.05.2025
Job Description:Trinity is Ireland's leading university and is ranked 87th in the world (QS World University Rankings 2025). Founded in 1592, the University is steeped in history with a reputation for excellence in education, research, and innovation, which has been inspiring generations of thinkers for over 400 years.
A part-time Research Assistant is required to support a DAFM (Department of Agriculture, Food and Marine) funded project entitled "Detect PNP: Detecting plant nursery pathogens using HTS technology". The project aims to provide methods for the detection of pathogens in plant nurseries using high-throughput DNA sequencing (HTS) technologies. Plant nurseries are normally inspected visually for pathogen collection and screening; however, this is time-consuming and relies on the presence of disease symptoms and the specialist knowledge of the inspector. To overcome these limitations, HTS methods offer high potential through eDNA application for pathogen detection and quantification.
We will sample wastewater, growing media, and plant material from nursery, botanic garden, and research environments and develop the HTS pipelines for long and short read sequence detection of pathogens. It will also use whole genome sequencing of core isolated and cultured target microbes to provide genomic reference tools for further characterization and screening. The focus is on hardy ornamental nursery stock and fungal and bacterial non-quarantine pathogens of Rhododendron and Prunus, including Phytophthora and Pseudomonas. This project will provide a more general methodology to apply these methods in routine pathogen surveillance, to guide inspection staff, and to understand the implications of this approach for the detection and regulation of plant pathogens.
The project will develop a roadmap on how the HTS technology could be used to augment plant health surveillance in nurseries and other ecosystems in Ireland. It is a collaboration between Trinity College Dublin, Teagasc, DAFM, and the National Botanic Gardens Glasnevin.
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